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11. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, separating the substance from the solution with ammonium suplhate, dissolving the ammonium sulphate from said substance, and concentrating said substance with a reagent which will unite with it as by adsorption.

12. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and fufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, separating the substance from the solution with ammonium sulphate, dissolving the ammonium sulphate from said substance and concentrating said substance with benzoic acid.

13. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, separating the substance from the solution with ammonium sulphate, dissolving the ammonium sulphate from said substance, concentrating said substance with benzoic acid, and separating said substance from the benzoic acid.

14. A method for obtaining a potent substance from