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said substance from the gland with a solvent, concentrating said substance with benzoic acid, and separating the substance from the benzoic acid.

8. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, concentrating said substance with benzoic acid, separating the substance from the benzoic acid, and making an aqueous sterile solution of said substance.

9. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with an acid solvent and concentrating it with a reagent which will unite with it as by adsorption.

10. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, separating the substance from the solution with a reagent as a solid, dissolving said solid, and concentrating said substance with a reagent which will unite with it as by adsorption.