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concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, concentrating it with a reagent which will unite with it as by adsorption, separating the substance and the reagent, and making a sterile aqueous solution of the substance.

5. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration to patients having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, filtering the extractive, separating said substance from the filtrate with a reagent which acts as by adsorption, and separating the substance and reagent.

6. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration having the hereindescribed physiological characteristics which consists of extracting said substance from the gland with a solvent, filtering the extractive, separating said substance from the filtrate with a reagent which acts as by adsorption, separating the substance and reagent, and making an aqueous sterile solution of said substance.

7. A method for obtaining a potent substance from the ductless portion of pancreatic or related glands in concentrated form and sufficiently free from impurities for repeated administration having the hereindescribed physiological characteristics which consists of extracting