of exhausted gland per rectum* There was no effect. At 8 a.m. Sejt.
lOfrfi, fifteen c,c« of extract of exhausted gland were injected intrgU
venously. The drop in blfcod sugar im. very marked* Twenty C4c. of px-
hausted gland extract,made .ijpy^alkaline with ITaoH,were incubate^
three hours at body temperature with 10 c%c- of active pancreatic juice,
This solution was neutralized and injected intravenously at 7 p.m?
September 10>H!i« No reduction in blood sugar resulted. At 2 £um.'
September llttf 20 c,c • of acid extract incubated for three hours at
37«5°P were injected. The curve shows the drop in blood sugar. On
September 131*K' at 9 a-m. and 2 p.m. the effect of extracts from ijhe
partially exhausted gland of a cat is shown. This extract produces
a pronounced/reaction.
We observe that extracts prepared from these more or leps
i
exhausted glands, while retaining to some extent the reducing effect
upon blood and urine sugar, produce many symptoms of toxicity whi|h
are absent after injections of extracts from completely degenerated
glands.
Chart Ho*, 5 is the graphic record of an experiment on a
10 ^QA^gifffn dog in which we have attempted to prove that the reduction
of the percentage sugar in the blood is not a dilution phenomenon^
Our plan of campaign was to inject a1?a given hour on the first day
(2 £.m.,0ct. 7ttf) , 100 cc. of isotonic saline(l). On the second day'
at the same hour the animal received 100 c.c- of lOJty^* sugar solution
(2). Extract (3)(j5) (10 and 15 cc, doses) was given one hour,and a
second dose thirty minutes before the corresponding hour on the third
day and 70 c,«. of distilled water containing 10 gms. of sugar werej
injected on the hour (4). Extract in 20 c.c. doses was injected at