Cyjs&*-
f< an extract of degenerated pancreas wfcre injected. A sharp fall inf
the blood sugar resulted* At 3 £*m» and again at 4 £»m. a similar doge
of extract was given. The chart records the lasting effect* The 2 g>m.
level of .30?^^was regained twelve hours *after the first injection* i "~
The hourly excretion of sugar ran approximately parallel with the pert
centa*ge of sugar in the blood. Between 1 s>*m. and 2 £.m* *52 grams
were excreted* Less than .02 grams were ex'cfeted between 7 p^.nu and
8 ;•!&• The highest glucose to nitrogen ratio observed in this experiment
was" a 3:1 value for the 22-hour interval between 2 2^m. the 6th of August
and noon the following day* At 12 noon August 61#l the percentage su^ar
in the blood was .40^^ Five c.c. of boiled extract of degenerated I
pancreas was injected intravenously./. Wn secured no reduction of blo4d
sugar* At twelve midnight August 61m five- ac- of extract of degener4ted
pancreas which had been prepared 48 hrs^weSs administered* The blo<|d
sugar fell from . 43^**at 12 £•*£* to .37|^f^at 1 a/m* Five c.c. dose$
were given at 1, 2 and 3 a*W- and a twenty-five ccf-^dose at 4 a.jn. fhe
chart shows the reduction'in blood sugar to a normal level and^tne f
beginning of an upward trend five hours after the last injection of ox-
tract. 5he animal died at 12 a.m. August 7frft.
A Brief description of * the clinical condition of the animal,
at various stages of the experiment ignecassary for the correct inter-
pretation, of the above results* The &$&$&% made a good posl£3>perat:.ve
was able to retain water and b«pe meat after,the second da;r
following'the operation* On the morning of August; 5WI we noticed tl.at
the condition of the animal J£as ^uo^^worse. j^Jt appeared excessively
tired, did not eat, and vorril^ecl/water^ .A/i^Mfel^$omitod after extract!
At 5 T>.^.j[August 5TO the animal appeared considerably I
c/ improved.'x It retain* water and ate meat. "• < On August 6i^f at 10 g*m.
the HMri^A*fck abdominal wound was moist with exudate •*• It was not so j
active as on the preceding day. No marked variatitm from tMs conditton
was observed until 4 a-m. when 25 cp. of extract were administered. 1
^ After this injection fhe' animal had a marked reaction and appeared to|
be dying- It was revived slightly by intravenous and intraperitoneal!'
injections of warm saline. Considerable improvement was noted at 7 aim.,
l5og was able to stand. The improvement was short-lived* The dog die*4
at 12 ^-m* August ^SWf. The postjmortern showed a widespread abdominalf
infection'* There was no sign of"pancreatic tissue-. ^|
The entire degenerated pancreas from one 8 *sM|p dog and I
approximately one-half the degenerated gland from a 6 ftaLo dog was thq
substrate of the extract used in this experiment. • (/ j-
Chart ^N^. 4, Dog 9 gives additional evidence on several im-
Ijportant points v;hich have been deferred to previously. At 6 ]3.m. ,
September 8WT. we administered ten cc« of extract of degenerated pan-,
creas per rectum* There was no reduction in blood sugar at 7 £.
7^jj-\&- we gave 12 cc. of extract of exhausted gland intravenously* Thle
chart records the effect of this and subsequent injections of the same]
material- At 6 a.m. September 10>n we administered 15 c.c- of extract