I *
nineteen *£k&0 dog by continued injections of secretin and repeated i
stimulation Tof the vagus nerve below the diaphragm. We obtainedf
eiflfty-five c.c* of pancreatic juice and considered the gland exhaustedj»
It was swiftly removed and immediately chilled. The marked effect of I
injection of this material is shown on the chart at 7 ^.m. August 20;fe&
On August 21at we incubated ten cc* of the extract and five c,c* of \._
pancreatic juice for two hours at body temperature in alkaline solutiofi
This solution was injected at 6 £.m. August 21sH5. The curve shows the!
very slight effect produced. As'a*control on the' above,ten o.c» of ex-f
tract and five cp. of saline were incubated under similar conditions I
for two hours. The chart at 10 g*m« August Ela^C records the marked /
effect of the injection of this second solution. On August 22j*d at
6 p^m* eight cc- of extract from the normal pancreas of a cat were
injected. We 'obtained a marked anaphylactic-like reaction. The curv
shows the effect upon the blood sugar.
. > No further injections were given to this animal after Augus
EE^.*Uid5pg died on August 30tfef nineteen days after the operation. Tl*e
autopsy'showed consolidation and necrosis of a large area in lower lol^e
of right lung, infection in right pleural sac. The operation wound |
was well healed* There was no sign of pancreatic tissue./Abdomen was
infected.A <\ .
3, is the record of dog 408. The weight of this
. The details of the experiment will be given
Eighteen
*'; animal was 9
VMJ
rather fully.
after/x
The normal blood sugar of dog 4p8 was
>n the percentage^eugar in the blood was
Twenty-two hours after the operational ]/.m. Aug.41^} , the blood suga?
was . 26JL^**£* During the twenty-two hours 3.10 grams of sugar were ex- \
creted.^ The volume of urine was 494 cc. At one £*m* we administered \
'V five QC* of extract of degenerated pancreas which had been prepared \
' four days previously and kept in cold storage* At two &.£!• the blood 1
sugar was .16ty^*j At three B-m* the percenttf*bf sugar in The blood had I
fallen to .15^*^ From one to * three &.m. . »19 grams of sugar were ex- j
creted in a volume of twenty-six c,c. of^urine. (3743) grams of urinary j
nitrogen were excreted in the twenty-four Jiours loTTowing the operatic^
The a. to IT. ratio for this period was 1*4 : 1. From 3 jo.ip. to 7 p_.m,' "
the percentage of sugar in the bloodj shows a gradual rise' from .15 %o*
•25'bXf^'* This latter level was maintained until 9 ^.m. The chart sho
a slight rise in sugar excretion following the rise 'oJ blood sugar. \
At 9 ]3;ij* five c c • of extract which had been exposed to room temperature
for one hour was* injected intravenously. The blood sugar was reduced ;
to a valuer of -ISf^v The chart show^a a gradual ascent from this value
to .27|^r At 10 /*m. the percentageYsugar in the blood was .27. At /
this hour five cc* "of extract of liver, prepared in precisely the same |
manner as the pancreatic extract, were administered intravenously. j
One hour later the blood sugar was .SOR^^*" This level was maintained \
during the following three hours. It was unaffected by an injection o:ff
5 ctc» of extract of spleen. The chart shows the rise in volume of |
urine and amount of sizgar excreted. At 2 ^.m.. tB.S. .3|*?tf*| five cj3. f