I
*of a £0 gin. inj ^jtion in a period of five hours following the injectipn,
in contrast to an excretion of 15.88 grams of a 25 gm. injection in tjie
same interval, when no extract was administered.?
% Chart Tfa*2 is the record of dog 92, weight 11.9 £0, A'^omplete i
pancreatectomy was performed on this animal at three p.m. August
The first injection of extract was given six hours after the operatiofi
and subsequently an injection every four hours. This extract was I
freshly prepared from a ten
been ligated for ten weeks
dog whose pancreatic ducts had |
JP!
One hundred and twenty-five cc. of e*tra6t
were prepared from the gland residue Irut this supply was exhausted by I
two p.m., August 13Ali. , after which other extracts were used. Blood (
•a. *i
samples were always taken before the injections of extract.f /\\
.
On August ISJ^y the blood sugar curve shows that neither five nojf
eight c,c. of this extract every four hours were sufficient to counter-j
balance the upward trend of the percentage of sugar of the blood. At!
10 p.m. the dose, was increased to twdlve cc. and a marked fall is note^d.
The "chart at 10 a.m. August 14£ft,r records 'the reduction of the percent-
age of sugar in the blood below its normal level, as a result of extract
from another degenerated gland. (The exceptionally higher values for :
the volume of urine and the urinary nitrogen for August 15JW1/ and 16:&V"
may be due to the adulteration of the urine with vomit.) On August 15|til
at 10 a.m. the chart shows the effect produced by ten cc. of the same I
gland extract made O.^acid with HCI. This extract made O.lf^)alkalinfe
with HaOH causes a slight reduction (August 15th., 8 p.m.). The effect
may be due to the alkali." * <
The extract administered at 10 a.m. August 16>tf/ was neutral and i
made from the .same degenerated gland? "'- I
On August lopfly and l?"^/ effects of extracts from normal glands I
waAt tested. A normal pancreas from u ten ttfe-ogyam animal wus divided/
into three equal parts. One^ third was extru'cued with neutral saline, v
the second portion with 0.1$) 'HCI and the third with O.l^S/HaOH. On |
August 17t-h..at four P.m. the neutral whole gland extract was admin- |
istered. A marked f all-in blood sugar resulted. The acid and alka- |
line extracts were injected at 1^ g.g. August 17^K; and 7 a.m. Aug- f
ust 18th. The last two injections 'were perhaps not given a fair J
opportunity to develope their effects. We do not take colorimeter |
readings by artificial light and therefore did not have an accurate J
knowledge of the height of blood sugar at these times.I
The conclusion from this experiment is that freshly prepared f
neutral or acid extracts of the whole pancreas do have a reducing I
effect on blood sugar, thus confirming llleiner. It may be stated hdre
that repeated injections of whole gland extracts cause marked thrombosis
of the Ire ins where the injections are made and u noticeable interference
with kidney function. It is obvious from the chart that the whole i
gland extract is much weaker than that from the degenerated gland. '
On August £0#y we attempted to exhaust the pancreas of a