The mortar was placed in freezing mixture and the contents partially
f$£zen. The half frozen gland was then completely macerated. The
solution was filtered through paper and the filtrate, having been
raised to body temperature, was injected intravenously.
We have never found it necessary to cut down on a vein under
general or local anaesthetic. The skin surface above the vein is
shaved and the needle inserted into the vein which is dilated by
compression* The dogs make very little resistance to this procedure
I
and after the first few punctures lie quietly during the operation*!
Sugar injections (100 ac. of fluid) as ^well as the numerous administr^-
tions of'extract were conducted by this method.
We e^ond^UQted several experiments with the object of exhau^t-
ing the zyrnogen granules of the pancreas* Prolonged sccretin in-
jections and vagus stimulation below the diaphragm were practiced.
Fortune favoured us in tke first experiment. In subsequent attemptp
we were never able to exhaust the gland sufficiently to obtain an
extract free from the disturbing effects of some constituent of pan
creatic juice-
The blood sugar estimations were made by the Myers-Bailey|
modification of the Lewis-Benedict method*. The results of this method
were corroborated by the Schaffer-HartmanXy^method at high and low •
percentages of blood sugar. The former method gave results which !
were consistently slightly higher { *Ol]|^<*Tthan those obtained by
the Schaffer-Hartman method. We find the average normal blood sugar^
from observations on thirtjr normal dogs, to be .090 12^*.j
Hemoglobin estimations were made by the carbon-monoxide !
saturation ~^csioi1 Imogen