.
I<**
anaesthesia an upper right rectus incision -we made through the ab-
'& •
dominal wall. The duodenum w»e delivered through the abdominal
~
pancreas ffl traced to the tail portion. /Mesentery beyond is cut
between clamp and ligature. Vessels from spleen are then isolated,
then dU-
ligated and divided. Little dissection is/required until/duodenum is
reached. The superior pancreatico-duodenal vessels are located and
great care is exercised to avoid damaging them. The pancreas is striked
from the duodenum by dry dissection. The vessels to the uncinate process
are ligated and divided, and the process freed from its mesenteric
attachments. The larger duct of the pancreas is then ligated close
to its entry into the duodenum and the pancreas is removed. Special i
care must be exercised to preserve the splenic vessels. The superior I
pancreatico-duodenal vessels must be lefj^-intact• Failing .this , duo-
denal ulcer is a frequent development. If this procedure is carried
out the whole gland with the exception of the portion in contact with ;
the duodenum is covered with mesentery. The abdominal wound is closed
layer by layer with catgut. A collodion dressing is used. The urethral
orifice is exposed by a midline incision of the perineum and the edges/
of the wound drawn together to facilitate healingj
We have found that animals between eight and sixteen months
old are the most suitable for this operation. At this age the pancreas
i6 not so firmly fixed as it/later,\becomes/ ^A*s
A ^—^I
We first ligated, under general anaesthesia, the pancreatic {
ducts in a number of dogs. (Blood sugar estimations on these animals
were recorded from time to time. We have no record of a hyperglycemia).
The extract was prepared as follows • The dog was given a
lethal dose of chloroform. The degenerated pancreas was swiftly re-
moved and sliced into a chilled mortar containing Ringer's solution.