11
When dog 27 died we were using Dog 33
as a trial dog for the effects of the various
forms of extract. This dog had been given
almost every variety of extract we have
prepared, and in every method of administration.
We had from time to time given therapeutic
doses and at the end of 20 days the dog was
in excellent condition. We then converted
her into a longevity experiment. A dose of
extract was given twice a day and later
once per day and the animal was fed on
lean meat, milk, and dog biscuits. A
slight gain in weight was noted. At the
end of 70 days the dog was becoming
thinner and weaker but was still able to
walk and wag her tail. The animal was
then chloroformed and an autopsy
preformed by Dr. Robinson. No islet
tissue whatever could be found.
However in the subnucous layer