68 SCIENTIFIC PROCEEDINGS (125)
without further treatment. iThese proved exceedingly toxic
causing extensive sloughing and ulceration of the tissues into
which they were injected, and sometimes even caused the death
of the dog. These wounds were sterile unless secondarily in-
fected. Simple alcoholic extracts Iikewise produced toxic ef-
fects, particularly when only small volumes of alcohol were used
and the trypsin was for this reason not wholly destroyed. Later
these toxic effects were very largely eliminated by purification
of the extracts.
Tables and graphs of experimental data were given showing
abrupt falls in blood sugar, disappearance of urinary sugar and
rise of the respiratory quotient following injection of extracts.
Three months study of the influence of the anti-diabetic substance
on a case of severe diabetes.
By C. CLYDE SUTTER and JOHN R. MURLIN.
[From the Physiological Laboratory of the University of Roches-
ter, Rochester, N. Y.]
Anti-diabetic substance, prepared at the University of Roches-
ter was given to a case of severe diabetes mellitus (adult) by the
duodenal tube, by mouth and by subcutaneous injections. Ac-
curate study was made to determine the potency of the pancreatic
extract, the dosage required and the most efficient and practical
method of administration.
Glycosuria had been present at least for five years and was
progressively increasing. Acidosis was constant during the past
year. At the time of beginning this study the elimination of
urinary sugar was greater than the intake of carbohydrate.
The patient was placed in the Rochester General Hospital,
July 6, 1922, and studied while on a weighed diet consisting of
carbohydrate 29.5 gms., protein 69 gms., fat 107.48 gms., and to-
tal calories 1394. During this study the blood sugar averaged
from 0.410 to 0.513; the uri,nary sugar averaged from 32 to 45
grams; and the acetone and diacetic acid was 2+.
Anti-diabetic extract was given fifteen times through the duo-
denal tube from July 12, 1922 to July 29, 1922 inclusive. The
blood sugar was reduced, reaching its lowest, 0.241 on July 26,