66 SCIENTIFIC PROCEEDINGS (125)
Influence of pancreatic perfusates upon the carbohydrate meta-
bolism of depancreatized animals.
By HARRY D. CLOUGH, ARTHUR M. STOKES, C. B. F. GIBBS, NEIL C.
STONE and JOHN R. MURLIN.
[Prom the Physiological Laboratory of the University of Roches-
ter, Rochester, N. Y.]
Following the method of perfusion introduced by Clark', the
effect of pancreatic perfusates upon the carbohydrate metabolism
of entire ainimals was studied. The respiratory metabolism, the
blood sugar, and the D :N ratio were studied in cats and dogs
Perfusates were made by perfusing aseptically and at body
temperature the pancreases of cats, dogs, pigs, and oxen with
Locke's solution. The first perfusates were made with an alka-
line medium, later perfusates were made with Locke's solution
modified by the substitution of HC1I varying in strength from
0.1 per cent. to 0.7 per cent. for sodium bicarbonate.
Respiratory quotients were obtained by a modified Jaquet
method, the animal being confined in a respiration chamber
through which a continuous stream of air was drawn for ventila-
tion and from which a continuous sample of air was removed for
analysis. In a later modification the stream of air from the
respiration chamber was directed into a large gasometer for a
twenty-minute period and the samples for analysis withdrawn
from this total volume. Analyses were made by the Henderson
and Bailey modification of Haldane's apparatus. The respiration
chamber was frequently checked by analyses of outside air and
by burning alcohol in it.
D :N ratios and respiratory quotients typical for diabetes were
obtained in the depancreatized animals.
Following the administration of sugar in the form of dex-
trose or sucrose by stomach tube pancreatic perfusates were given
subcutaneously, intravenously, and intraperitoneally. Significant
increases were shown in the respiratory quotients and marked
1 Clark, Admont H., Jour. Exp. IMecd., 1916, xriv., 621.
Ibid, 1917, xxvi, 721.