58 SCIENTIFIC PROCEEDINGS (63).
on a regular slide for study with dark field illumination. In
essentially the same way pieces of tissue were placed in plasma
under a cover glass and sealed. Precautions to secure aseptic
conditions were taken.
This method keeps trypanosomes living, growing and dividing,
and thus many of the stages described by various authors either
in the host itself or in the transmitter have been studied in vitro.
It is evident that this method may be employed not only for blood
parasites but for all protozoan forms which are parasites in cells
and thus affords another method of approach for the solution of
the complicated life cycles of parasitic Protozoa.
The influence of depancreatization upon the state of glycemia
following the intravenous injection of dextrose in dogs.
By I. S. KLEINER and S. J. MELTZER.
[From the Department of Physiology and Pharmacology of the Rocke-
feller Institute for Medical Research.]
In former experiments' it was shown that after the intra-
venous injection of large amounts of dextrose (4 g. per kilo) into
dogs the sugar rapidly disappears from the blood stream so that
after I 3 hours after the end of the injection the blood-sugar falls
nearly to its original figure. In the present experiments the same
procedure was carried out on completely depancreatized dogs.
In these cases the blood-sugar did not fall to its original value or
near it; at the end of Is hours it was on the average more than
twice as high. The following is a comparison of the average
Dextrose in Urine,
fore Enod of I Hours After PerCent. of Amount
Before End of ~~End of Injected.
Injection. Injection. Injection. j
Normal (5) 0.20 0.79 0.27 >43%
Depancreatized (9). 0.38 I.I9 0.86 49%
"diabetic " sugar).
i See Proceedings of the American Physiological Society, Vol. 33, I9I3, p. xxvii.