BLOOD PRESSOR SUBSTANCES IN IMMUNITY. IO3
temperature was at all times kept below 500 C. instead of being
allowed to go to 65° as before. So far five cats have been injected
with the extract, the first three with two injections each and the
others with one each. All injections were made beneath the skin
of the back. The results are shown in Table III and are calculated
to grams of glucose per hundred grams of blood as in Table I.
The average for the injected cats is over 2I per cent. above that
for the normal ones, and moveover the amount of sugar is greater
in each injected cat than for any normal animal except the one
that gave 76 mgm. This increase is very surprising and of peculiar
interest. At present I am not willing to venture any explanation.
There are however several possibilities which are amenable to
experiment and I hope that further work will throw some light
on it. In any case it would seem to put in grave doubt the idea
that the pancreatic hormone always tends to increase the storage
of glycogen in the liver at the expense of sugar in the blood.
There are a number of factors entering into the experiments
so far performed that might cause the individual variations in the
experimental results. Some of the more probable are: the length
of time intervening between the injection and the death of the
animal; the amount of extract injected; the age of the extract;
the number of injections and the time intervening between them,
etc. At present I am trying to find some of the optimum condi-
On some blood pressor substances and adrenal separations
in experimental imnmunity.
By J. P. ATKINSON and C. B. FITZPATRICK.
One of us in a work' on "The Preparation of Diphtheria
Antitoxin" endeavored to demonstrate by charts of the systemic
reaction following injections of cultures of the bacillus of diphtheria
and its toxin that the real crux of the process of immunization was
to determine when to re-inject. This question is still unsettled;
in short, of two animals treated the same, upon being re-injected
the one, which may apparently be the better prepared, dies and
the other recovers.
I'Fitzpatrick, N. Y. Medical Journal, April 27, I895.