I02 SCIENTIFIC PROCEEDINGS (52).
mining the amount of sugar Munson and Walker's "Uniform
method of sugar determination" was used and gave consistent
results. In order to test its efficiency the method was controlled
by division of a sample of blood into two portions. In one of
these the glucose was estimated directly. To the other a known
amount of glucose was added and then this amount was subtracted
from the total recovered, leaving a remainder which should equal
the amount found in the portion estimated directly. The results
of one such control are shown in Table I.
Blood sample 6 (two cats). Total blood uscd I0o.o5 gm.
a b c d
Gm. blood in sample ................. 47.5I 47.5I 47.5I 47.5I
Gm. glucose recovered . .............. o.o3I4 0.0328 o.o5oI 0.0497
Gm. glucose added .7..........8 O.O178
Gm. glucose in blood . ................ o.o3I4 o.o328 0.0323 o.o3g19
Gm. glucose per 0oo gm. blood........ o.o66z 0.0690 o.o680 o.o67I
Average for ab and cd................ o.o676 0.o676
It was necessary next to determine the amount of sugar
in the blood of normal cats. The results for a series of ten cats
are given in Table II. It will be noticed that with the exception
of the cat which gave 76 mgm.-obviously an anomalous case-
the greatest variation from the average is 4 mgms. The starred
numbers indicate controls such as are reported in Table I. The
results are calculated to grams of glucose per hundred grams of
TABLE II. : TABLS III.
o.o676 * o.I0o26
o.o676 j o.o783
o.o628 Average .... o.o806
The extracts of the pancreas that were used in the following
experiments were prepared as reported previously, except that the