Lydia M. DeWitt 233
of the possible occurrence of glycosuriacl after the first month,
I did not continue the examinations long enough to be able
to speak upon this point. In four of my cases, I tried the MSayo-
Robson test on the urine just before death, with positive results
in three cases and negative in one.
In all cases except when spontaneous death occurred during
the night, the pancreas was removed immediately after the
death of the animal and small pieces taken, one from the liga-
tured end, one from the neighborhood of the ligature, and one
from the free end, and fixed in corrosive-sublimate solution for
microscopic study. The rest of the gland, usually in two portions,
was extracted either with glycerine or with water and its diges-
tive and glycolytic powers tested. Starch digestion was tested
with iodin and with Fehling's solution. Fibrin digestion was
determined by the use of the bromine tryptophan reaction and
the biuret test. Fat digestion was indicated by the reaction
and by emulsification. The glycolytic power was tested by
following as nearly as possible Cohnheim's method: a definite
amount of glucose was added to a definite amount of muscle
extract, and to this were added the pancreatic extract and also
large amounts of toluol for its bactericidal action. In some
cases, both boiled and unboiled pancreatic extracts, were used
vith the muscle extract and with the same results. The quan-
tity of sugar was estimated, at once after mixing and again
after twenty-four hours in the warm oven, with the ammoniacal
copper solution of Pavy. Control tests with muscle alone and
with pancreatic extract alone were carried on at the same time.
The results of these various tests are briefly indicated in the following table
in which O indicates the mental end (usually the ligatured end), while D in-
dicates the duodenal end (the free and hencemore normal end), of the pancreas.
It will he noted from this table that seven of the twenty cases in which physio-
logic tests were made (and this included six of the ten cases in which the gland
tissue was nearly normal) showed no digestion of starch, fibrin, or fat, .while in
several others the digestive action was very much weakened. The diminution
and absence of digestive action are proportionate to the atrophic condition of
the glandular tissue. In none of the cases, however, was there any appreciable
weakening of the glycolytic or activator powver of this extract, even when the
glandular tissue was apparently atrophied