I.S. Kleiner 161
sugar because the next urine obtained by catheter contained
3.62 per cent dextrose.
On the following day (see DPlb in Table V) an injection of
another pancreas preparation lowered the blood sugar from 0.27
to 0.20 per cent. The urinary sugar excretion was diminished
very markedly; in one period no qualitative reaction whatever
could be obtained.
Experiment LP81a.-In Table IV is given an outline of Experi-
ment LP81a, one of the control experiments. The animal received
an intravenous injection of submaxillary gland emulsion.. The
slight decrease in the blood sugar percentage is in marked contrast
with the results obtained when pancreas emulsion was used, and
-uns parallel with the hemoglobin; i.e., here what little effect there
is may be referred to dilution by the intravenous injection of 114
cc. of fluid. The urinary data are insufficient in this experiment
but indicate a possibly diminished output of sugar.
There were no noticeable general effects caused by the infusion
and on the next day the administration of pancreatic emulsion
lowered the blood sugarfrom 0.38 to 0.24 per cent (see LP81b, Table
V).
In Table V all the experiments with pancreas emulsions are
collected. It will be seen that in most of them there occurred
a substantial reduction in the blood sugar. As the samples were
not taken at short intervals it is quite probable that the lowest
values were not discovered. However, normal or nearly normal
figures were found in several cases. Study of the table indicates
that, when effective, the emulsion produces an effect during the
slow injection, because the blood sugar is usually lower at the
end of the injection than at the beginning. The glycemia remains
low or continues to fall for about 1½ to 14 hours, and then, if we
may trust the few figures at hand, it gradually rises. It is certain
that the effect does not last many hours, for in a number of the
experiments a sample of blood was taken on the following morning
and invariably showed a hyperglycemic value.
In all except one experiment (LP75), there occurred a dimi-
nution in the excretion of sugar. In some cases this was quite
marked, resulting in a urine reacting negatively to Benedict's
qualitative test. Such urines, however, had normal color and
specific gravity. In about half the experiments there was a
TFH] JOURNAL OF BIOLOOICAL CHEMISTRY, VOL. XL, NO. 1