156 Pancreas Emulsions in Experimental Diabetes
the blood was drawn directly from an external jugular vein into
a syringe. The urine was obtained by catheter in all cases.
Analytical Methods.--The blood sugar determinations were
made by the Myers-Baileyl° modification of the Lewis-Benedict
method. Glucose in the urine was estimated by a modification
of the Pavy" method or by Benedict'sl2 method. Hemoglobin
was determined in most of the experiments by discharging 2 c.
of blood into dilute HC1 (about 0.3 per cent) in a 250 cc. volu-
metric flask, diluting to this volume, and comparing the density
of the color in a Duhoscq colorimeter. The first blood sample of
each experiment was used as the standard and was taken as 100
per cent for that experimnent. Later the Sahli hemioglobinometer
was used and, to avoid confusion, the results are similarli
Four illustrative protocols, including three injections of pancrens
emulsion and one control, follow.
Experiment LP72a.-From Table I it will be observed that tle
high blood sugar value of 0.31 per cent feU to 0.14 per cent in tle
course of the 78 minute injection period and after another period
of 90 minutes it was still low (0.13 per cent). Both of these figures,
0.14 and 0.13 per cent, are practically normal values, but emphasis
is to be placed on the extent of the drop, from 0.31 to 0.13 per
cent, a fall of 0.18 per cent, rather than upon the absolute valiue
finally obtained. At the same time this decrease in the concen-
tration of the blood sugar is not accompanied by any change in
the hemoglobin value, or by an increased glycosuria. In other
words it is not due to a dilution of the blood sugar by the injection
fluid, or to a washing out of the sugar through the kidneys. In
fact the kidney excretion of sugar is greatly diminished.
This was one of the few experiments in which the injection pro-
duced any noticeable general symptomns; there was first a fall and
then a rise in temperature. There was no marked harmful effect
of the infusion, however, as the animal was given a second infusion
2 days after the first and lived 2 days after that. The second
infusion caused only a slight fall in the glycemia (see LP72b in
t0 Myers, V. C., and Bailey, C. V., J. Biol. Chemrn., 1916, xxiv, 147.
" To be published shortly.
' Benedict, S. R., J. Biol. Chem., 1911, ix, 57.