I. S. Kleiner 155
itroduce the pancreatic preparation by the intravenous route very
.!owly and over an appreciable period; the results, however, might ·
!e expected to be observed during the injection and for only a few
ours thereafter. These principles were followed and the results
< re very gratifying-in a word, the slow intravenous infusion
,f an aqueous pancreatic "emulsion" usually resulted in a tem-
oraryy but marked decrease in the glycemia and glycosuria. The
e;st few experiments were briefly reported in 1915.6 The present
,aper includes these experiments as well as a large number of
xpcrrirnents performed since the first communication..
Method.
Operation.-Total depancreatization was performned in the
*ajority of cases. In three animals, used in the six most recent
xperiments, the "Allen" procedure, i.e. leaving a small remnant
:round the large pancreatic duct, was adoptecd. The operations
were performed under ether anesthesia.
Preparation of "Emulsion".-Fresh dog's pancreas was hashed,
«ixed with three or four times its weight of sterile distilled water,
:!nd placed in the refrigerator. After a period of'from i to 20
i«ours it was strained and squeezed through muslin. The fluid
tbus obtained was diluted with 5 volumes of sterile 0.9 per cent
NaC1 solution before injection. This dilute solution was faintly
:<id or neutral to litmus, light pink in color, and almost clear. At
«o stage was it filtered, nor was sodium carbonate or any other
'obstance added. In one experiment (LP77a),Ringer's solution
\-as used in the extraction arid also in the dilution; this did not
,( ad to a better result. The emulsions of other tissues, used in
lie control experiments, were prepared in a similar manner.
Injection and Blood Sampling.-Some time before the infusion
,as to be given, the animals (in most of the experiments) re-
e'ived a small dose of morphine sulfate subcutaneously, usually
<)out 1.5 mg. per kilo. Cannulas were then introduced under
'<al anesthesia into a convenient vein and artery, for injection
«itl blood sampling, respectively. In the six recent experiments
o, morphine was given and only one cannula (the venous) was
;itroduced under local anesthesia (ethyl chloride). In this series