Murlin, Clough, Gibbs, and Stokes 281
and illustrated a fact whichwe have repeatedly confirmed; namely, that the
second extraction made in this way contains more insulin than the first.
The blood sugar declined from 0.310 to 0.041 in 6 hours from the time of the
first injection. From a D:N ratio in the early morning of 0.56 the glycosuria
had completely disappeared within the same time and remained absent
over night. On Aug. 17 the dose was reduced to 25 cc. and at 2 p.m. 20 gm.
of dextrose were given for the purpose of a respiration experiment which
because of a temporary defect in the apparatus was not performed. The
sugar had overwhelmed the effect of this one dose of extract and as a con-
sequence the blood sugar rose. Sugar also appeared in the urine, but up
to 9.30 a.m. the next morning only 8.52 gm. of the 20 gm. fed had been
excreted and at 9.50 a.m. a more effective dose of extract began to check the
excretion 80so that only a negligible amount of sugar escaped. It is fair to
state that the 25 cc. of extract given at 10 a.m. on Aug. 17 had disposed of
(not necessarily caused the combusion of) some 12 gm. of glucose. The
single dose of 50 cc. given at 9.50 a.m. on Aug. 18 was sufficient to hold the
urine sugar-free throughout the day. It is worthy of remark that Dog 7
lived 41 days after pancreatectomy and finally died by accident.
The only special pertinence of the foregoing experiment as
against any one of several others of like purport which might be
reported is that these two preparations, Nos. 62 and 63, were
later cleared of protein, concentrated, and admninistered to a human
case (26) of diabetes with complete control for the time of the
glycosuria and hyperglycemia.
The remainder of this paper will be devoted to the presentation
of additional evidence that extracts prepared in acid aqueous
media can bring about the oxidation of glucose. The only differ-
ence between the experiments already cited in proof of this propo-
sition and those to follow is, that in the earlier group the extract
employed was made from pancreas of the identical species, while
in the latter, made entirely from pancreas of beef, it was used exclu-
sively on the diabetic dog.
It is not necessary here to enter into a discussion of the respira-
tory quotient of diabetes. That ground has been covered suffi-
ciently in previous papers (23). The quotient with which one
prefers to start is that which is typical of total diabetes; namely,
0.68 or 0.69, as illustrated in Tables I and II. However, it is
not absolutely necessary to secure this quotient in order to
demonstrate a considerable rise following the administration of