INSULIN AND GLYCOLYSIS 471
(c) 5 cc. muscle juice; 5 cc. phosphate; 10 cc. normal saline; 3 drops 10 per cent
dextrose.
The mixtures were immediately analyzed and then incubated at 385°C.
Analyses
GLUCOSE PER 100 CC.
TIME
(a) (b> (c)
mDm. mrngm. mgm.
12:15 p.m. 118 209 173
1:45 p.m. 96 212 165
2:45 p.m. (103) 217 168
4:45 p.m. 87 218 189
5:45 p.m. 89 196 192
7:20 p.m. 67 185 177
8:20 p.m. 56 156 156
9:20 p.m. 56 156 130
10:20 p.m. 34 62 ?
Glycolysis was decidedly more rapid in blood alone (a) than in blood
plus muscle juice (b) or in muscle juice alone (c). Up to 5 hours after
the start of incubation there was practically no glycolysis either in blood
plus muscle juice or in muscle juice alone, and after this time the
glycolysis which clid occur il these fluids may have been due to bac-
teria. The unexpected results of this experiment led us to repeat
Cohnheim's observations according to his directions with the results
described in the next experiment.
September 25th. Rabbit 1 was given 4 cc. insulin at 10:30 a.m. and killed at
12 noon. The blood sugar in the defibrinated blood was 63 mgm. per 100 cc.
Normal rabbit 2 was killed at 10:45 a.m., the blood obtained and defibrinated,
and muscle juice prepared by Buchner's process. These were kept in the ice-
chest until ready. The pancreas of this rabbit (2) was thrown into boiling water,
evaporated almost to dryness; alcohol was added, filtered off, evaporated to dry-
ness and taken up in distilled water (Cohnheim). The following mixtures were
made:
(a) 10 cc. blood from rabbit 2; 4 cc. muscle juice; 4 cc. usual phosphate mix-
ture; 2 drops 10 per cent dextrose solution.
(b) 10 cc. blood from rabbit 2; 4 cc. muscle juice; 4 cc. phosphate 2 cc. insulin;
2 drops 10 per cent dextrose solution.
(c) 10 cc. from rabbit 2; 4 cc. muscle juice; 4 cc. phosphate; 2 drops 10 per cent
dextrose solution; pancreas preparation described above.
(d) I0 cc. blood from rabbit 1;2 cc. muscle juice;2 cc. phosphate;2 drops 10 per
cent dextrose solution.
(e) 10 cc. saline; 2 cc. muscle juice; 2 cc. phosphate; 2 drops 10 per cent dextrose
solution.