which the preparation of this latter extract depends is the same as that of
E. L. Scott5 and the favorable results led us to see whether adult pancreas
could be used in place of fetal. Six c.c. of whole gland extract prepared as
above were therefore injected daily from December 8 to January 3 inclusive.
On January 4 the administration of extract was discontinued and on this day
the dog excreted 12.86 grams of sugar in a volume of 450 c.c. of urine. The
animal was in good condition and was on a diet of lean meat. On January
5 it was not so hungry or so lively as on previous days and 4 grams of sugar
in 350 c.c. of urine were excreted. On the next day (January 6) 3.5 grams of
sugar and 320 c.c. of urine were excreted and the animal was in a very poor
condition compared with that of a few days previously. On January 6 the
diet was changed to one of milk and biscuit along with meat, and the sugar
excretion rose to 25 gm. At noon on January 7, while on the same diet, 8 c.c.
of whole gland extract were injected and the twenty-four hours' sugar excre-
Fig. 1. Fig. 2.
Figs. 1 and 2.-Dog No. 33 nine weeks after total pancreatectomy.
tion was found to be 22 gm. A further injection of 10. c.c. of extract at 9 A. M.
on January 8 brought the sugar excretion down to 2.0 gm.
The original weight of the animal (33) was 10.1 kgm., and one week after
the pancreatectomy it was 8.1 kgm. This was maintained fairly constant
throughout the second and third weeks after the operation and then slightly
increased. From the fifth to the ninth week (see Figs. 1 and 2), during
which time daily injections of 6 c.c. of whole gland extract were given, the
condition remained good and was so on the 63rd day when, to convince
ourselves that the extract was necessary to the health of the animal, we
discontinued its administration for three days (21, 22 or 23 of January) with
the result that the dog became so weak that it was barely able to stand. Ex-
tract was again given on the 24 and 25 of January with decided improve-
ment. On January 27 when the dog weighed 7.9 kgm. it was killed by an
overdose of chloroform and a careful autopsy immediately made by Dr. W.
L. Robinson, Pathologist at the General Hospital, Toronto. His report is as
follows:
_ 7