DOo 27-PANCREATECTOMY NOVEMBEB 14-WEIGHT 5 KILOGBAMS
DATE HOUR BLOOD SUGAR EXTRACT SUGAR EXCRETED WEIGHT
Nov. 15 10 A. M. .11 - - 5.0 k. (Total pan-
Nov. 14 3 P. M. .28 - - -
Nov. 17 8.30 A. M. .30 5 c.c. 12 noon 16 to 4.1 k.
9.15 A. M. .20 - 9 A. ar. 17 -
10 A. M. .17 5 c.c. 4 gins.
6 P. M. .15 5 c.c.
Nov. 18 9 A. M. .175 10 c.c. Previous 24 hr. 4.1 k.
10 A. M. .08 - vol. of urine
6 r. M. - 10 c.c. 100 c.c., no sugar.
Nov. 193 9 A. M. .21 - Previous 24 hr. 4.2 k.
6 P. M. - 10 c.c. vol. of urine 225
c.c. sugar free
Nov. 20 10 A. M. .20 10 c.c. 4.3 k.
Nov. 21 10.15 A. M. .26 10 c.c.
Nov. 22 9 A. M. .25 12 c.c.
Nov. 23 9 A. M. - 12 c.C.
Nov. 24 9 A. M. - 10 C.C.
Nov. 25 9 A. Mr. - 10 C.c.
Nov. 28 6 r. M. - 2 c.c.
Nov. 29 6 P. M. - 5 c.e.
Dec. 2 11 A. M. .15 - Previous 24 hr. 4.0 k.
12 A. M. - 6 c.c. vol. 320 c.c.
.5 gms. sugar.
Dee. 4 12 A. M. - 6 C.C.
Dog 27 was temoved from the metabolism eage on November 20. Sugar excretion was
not followed in the interval between November 20 and December 1. On November 20 the
animal was in excellent condition which was maintained until December 2. At noon Decem-
ber 2, six c.c. of fetal calf extract prepared 16 days previously was injected subcutaneously.
This extract had been prepared under aseptic conditions and immediately after its prepara-
tion was found to have a very low bacterial count. The solution was kept in a refrigerator
some distance away from the ice. At 3:30 r. r. December 2, the dog showed symptoms of
aI peculiar nature. Periods of unconsciousness with convulsive twitchings, retraction of the
head, salivation and frothing alternated with periods of semi-consciousness. These symp-
toms lasted from 3:30 r. ar. to 6 P. M., after 6 P. M. the animal gradually improved and
at 8 P. M. was resting quietly. On December 4 at 12 A. M., 6 C.C. of the same extract was
again given. At 1:30 P. M. the animal began to exhibit symptoms similar to but more
severe than those noticed on December 2. The dog died during the night of December 4.
Postmortem examination showed that all the pancreas had been removed and there was no
evidence of infection. Twelve c.c. of the same extract as was used in the latter part of this
experiment injected into a normal dog produced no apparent reaction.
1921. Daily sugar excretion in grams and 24 hour urine volume are clearly
shown in the chart, from which it will be seen that in twenty-four hours after
the operation (November 19, 10 A.M.), the percentage of sugar in the blood
was 0.33 per cent. Ten c.c. of filtered fetal calf extract were injected intra-
venously and one hour later the blood sugar was 0.17 per cent. At 7 P.M.
November 19, the blood sugar was 0.37 per cent when 10 c.c. of extract that
had been passed through a Berkefeld filter were injected. At 8 P.M. the blood
sugar was 0.26 per cent. At 9 A.M. November 20 the blood sugar was 0.37
per cent when ten c.c. of neutral Berkefeld extract which had been kept in
a water-bath at 78° C. for thirty minutes were injected intravenously without
causing any change in blood sugar. From this and other experiments we
believe that the active principle of this extract is destroyed by heating it to